Preface
Safety rules in the biochemistry laboratory
Seminar Amino acids: Proteinogenic amino acids
Seminar Proteins І: Structure and function of proteins
Seminar Proteins ІІ: Globular and fibrous proteins
Practical works: Proteins
Determination of the isoelectric point (pI) of casein
Determination of hemoglobin concentration by Drabkin's reagent
Seminar Enzymes I: Enzymes as biocatalysts. Nomenclature and classification. izoenzymes. Water soluble vitamins as cofactors. Kinetics of enzyme action
Seminar Enzymes II: Regulation of the enzyme activity. Reversible and irreversible inhibition of enzymes. Allosteric enzymes. Constitutive and inducible enzymes
Practical works: Enzymes
Determination the absolute specificity of urease
Calculation of Km and Vmax by Lineweaver-Burke method
Seminar Bioenergetics I: Macroergic compounds. Biological oxidation. Oxidative phosphorilation. Structure of the respiratory chain
Seminar Bioenergetics II: Short electron-transport chains. Free-radical oxidation and antioxidant defence
Practical works: Bioenergetics
Detection of cytochrome c oxidase and peroxidase in muscle tissue
Quantitative determination of total polyphenol content in plant extracts by Folin-Ciocalteu method
Seminar Metabolism of carbohydrates I: Digestion of carbohydrates. Transport of monosaccharides in cells. Glycolysis
Seminar Metabolism of carbohydrates II: Degradation of pyruvate. Tricarboxylic acid cycle. Pentose phosphate Pathway
Seminar Metabolism of carbohydrates III: Gluconeogenesis. Glycogenesis. Glycogenolysis
Seminar Metabolism of carbohydrate IV: Metabolism of mannose, fructose, galactose and lactose
Seminar Metabolism of carbohydrates V: Glycosaminoglycans. Glycoproteins
Seminar Metabolism of carbohydrates VI: Summary on carbohydrates metabolism
Practical works: Metabolism of carbohydrates
Alcoholic fermentation
Blood glucose curves of a healthy individual and a patient with type 1 diabetes
Determination of fructose in urine by Seliwanoff's reagent
Seminar Metabolism of lipids I: Dietary lipiids digestion. Transport of exogenous lipids
Seminar Metabolism of lipids II: Lipolysis. Degradation of even and odd numbered fatty acids. Synthesis and use of ketone bodies
Seminar Metabolism of lipids III: Biosynthesis of fatty acids and triacylglycerols. Endogenous lipids in plasma
Seminar Metabolism of lipids IV: Biosynthesis of cholesterol, bile acids and bile salts. Cholesterol Transport in blood - role of HDL and LDL
Seminar Metabolism of lipids V: Complex lipids and eicosanoids
Practical works: Metabolism of lipids
Urine reagent strip test for ketone bodies
Quantative determination of triacylglycerols in serum
Quantative determination of total cholesterol in serum
Seminar Metabolism of amino acids I: Digestion of dietary proteins. Transamination of amino acids. Oxidative and nonoxidative deamination of amino acids
Practical work: Metabolism of amino acids
Quantitative determination of alanineaminotransferase (ALT) in serum
Seminar Metabolism of porphyrins: Heme biosynthesis and degradation. Porphyrias. Hyperbilirubinemias. Iron metabolism
Practical work: Metabolism of porphyrins
Quantitative determination of total and direct bilirubin in serum
Seminar Metabolism of nucleotides II: Biosynthesis and degradation of pyrimidine nucleotides. Biosynthesis of desoxyribonucleotides
Practical work: Metabolism of nucleotides
Determination of uric acid content in serum
Seminar Nutrition and metabolism of xenobiotics: Metabolism of xenobiotics and endobiotics. Nutrition
Seminar Calcium metabolism: hormones that regulate calcium and phosphate metabolism
Seminar Intercellular signaling I: General principles of intercellular signaling. Intracellular receptors. Receptors - ion channels
Seminar Intercellular signaling II: G-proteiN coupled receptors. With intrinsic enzimatic activity
Seminar Molecular biology: replication, transcription, translation. Cycle regulation. Oncogenes and tumor-suppressor genes. Regulation of gene expression
Recommended literature
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