A Handbook of Biochemistry

Second Edition. Review Study Questions and Laboratory Manuals - A Clinical Approach.

Biochemistry courses provide students the knowledge and skills needed for future professional career in medicine and for research in the biotechnological and pharmaceutical industry.

The purpose of A Handbook of Biochemistry. Review Study Questions and Laboratory Manuals - A Clinical Approach is to serve as a resource to enhance student learning of theories, techniques, and methodologies practiced during biochemistry teaching and in the research laboratory.

МУ Проф. Д-р Параскев Стоянов
Варна, 9002
ул. Марин Дринов 55
press@mu-varna.bg

Preface
Safety rules in the biochemistry laboratory
Seminar “Amino acids”: Proteinogenic amino acids
Seminar “Proteins І”: Structure and function of proteins
Seminar “Proteins ІІ”: Globular and fibrous proteins
Practical works: Proteins
Determination of the isoelectric point (pI) of casein
Determination of hemoglobin concentration by Drabkin’s reagent
Seminar “Enzymes I”: Enzymes as biocatalysts. Nomenclature and classification. izoenzymes. Water soluble vitamins as cofactors. Kinetics of enzyme action.
Seminar “Enzymes IІ”: Regulation of the enzyme activity. Reversible and irreversible inhibition of enzymes. Allosteric enzymes. Constitutive and inducible enzymes.
Practical works: Enzymes
Determination the absolute specificity of urease
Calculation of Km and Vmax by Lineweaver-Burke method
Seminar “Bioenergetics І”: Macroergic compounds. Biological oxidation. Oxidative phosphorilation. Structure of the respiratory chain
Seminar “Bioenergetics ІІ”: Short electron-transport chains. Free-radical oxidation and antioxidant defence
Practical works: Bioenergetics
Detection of cytochrome c oxidase and peroxidase in muscle tissue
Quantitative determination of total polyphenol content in plant extracts by Folin-Ciocalteu method
Seminar “Metabolism of carbohydrates І”: Digestion of carbohydrates. Transport of monosaccharides in cells. Glycolysis
Seminar “Metabolism of carbohydrates ІІ”: Degradation of pyruvate. Tricarboxylic acid cycle. Pentose phosphate Pathway
Seminar “Metabolism of carbohydrates ІII”: Gluconeogenesis. Glycogenesis. Glycogenolysis
Seminar “Metabolism of carbohydrates” ІV: Metabolism of mannose, fructose, galactose and lactose
Seminar “Metabolism of carbohydrates V”: Glycosaminoglycans. Glycoproteins
Seminar “Metabolism of carbohydrates VI”: Summary on carbohydrates metabolism
Practical works: Metabolism of carbohydrates
Alcoholic fermentation
Blood glucose curves of a healthy individual and a patient with type 1 diabetes
Determination of fructose in urine by Seliwanoff’s reagent
Seminar “Metabolism of lipids I”: Dietary lipiids digestion. Transport of exogenous lipids
Seminar “Metabolism of lipids II”: Lipolysis. Degradation of even and odd numbered fatty acids. Synthesis and use of ketone bodies
Seminar “Metabolism of lipids III”: Biosynthesis of fatty acids and triacylglycerols. Endogenous lipids in plasma
Seminar “Metabolism of lipids IV”: Biosynthesis of cholesterol, bile acids and bile salts. Cholesterol Transport in blood – role of HDL and LDL
Seminar “Metabolism of lipids V”: Complex lipids and eicosanoids
Practical works: Metabolism of lipids
Urine reagent strip test for ketone bodies
Quantative determination of triacylglycerols in serum
Quantative determination of total cholesterol in serum
Seminar “Metabolism of amino acids I”: Digestion of dietary proteins. Transamination of amino acids. Oxidative and nonoxidative deamination of amino acids
Practical work: Metabolism of amino acids
Quantitative determination of alanineaminotransferase (ALT) in serum
Seminar “Metabolism of porphyrins”: Heme biosynthesis and degradation. Porphyrias. Hyperbilirubinemias. Iron metabolism.
Practical work: Metabolism of porphyrins
Quantitative determination of total and direct bilirubin in serum
Seminar “Metabolism of nucleotides ІІ”: Biosynthesis and degradation of pyrimidine nucleotides. Biosynthesis of desoxyribonucleotides
Practical work: Metabolism of nucleotides
Determination of uric acid content in serum
Seminar “Nutrition and metabolism of xenobiotics”: Metabolism of xenobiotics and endobiotics. Nutrition
Seminar “Calcium metabolism”: hormones that regulate calcium and phosphate metabolism
Seminar “Intercellular signaling I”: General principles of intercellular signaling. Intracellular receptors. Receptors – ion channels
Seminar “Intercellular signaling II”: G-proteiN coupled receptors. With intrinsic enzimatic activity
Seminar “Molecular biology”: replication, transcription, translation. Cycle regulation. Oncogenes and tumor-suppressor genes. Regulation of gene expression
Recommended literature